By Matthew D. Weitzman, R. Michael Linden (auth.), Richard O. Snyder, Philippe Moullier (eds.)
Today, development in rAAV-mediated gene move is so powerful that long term, effective, and regulatable transgene expression is reproducibly accomplished in huge animal types. The complexity of gene move brokers within the context in their medical use calls for investigators from a large choice of backgrounds to have an figuring out — or no less than an appreciation of — the regulatory surroundings and constraints that have an effect on vector layout, production, pre-clinical trying out, and medical use, with an emphasis on sufferer safety. In Adeno-Associated Virus: tools and Protocols, specialists from the U.S. and Europe have contributed present wisdom of this multi-dimensional box with regards to the biology of AAV, rAAV vector layout, vector production and product checking out, functionality of rAAV vectors in significant organs, rAAV-related immunological matters, layout of animal and medical stories, and medical event. Written within the profitable Methods in Molecular Biology™ sequence layout, chapters contain introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, with ease reproducible protocols, and notes on troubleshooting and warding off identified pitfalls.
Authoritative and available, Adeno-Associated Virus: equipment and Protocols offers an entire and accomplished figuring out of this multi-disciplinary and speedily progressing field.
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Extra resources for Adeno-Associated Virus: Methods and Protocols
1 22. Warrington, K. , Gorbatyuk, O. , Harrison, J. , Opie, S. , and Muzyczka, N. (2004) Adeno-associated virus type 2 VP2 capsid protein is nonessential and can tolerate large peptide insertions at its N terminus, J Virol 78, 6595–6609. 23. , and Chapman, M. S. (2002) The atomic structure of adenoassociated virus (AAV-2), a vector for human gene therapy, Proc Natl Acad Sci USA 99, 10405–10410. 24. Nam, H. , Lane, M. , and Agbandje-McKenna, M. (2007) Structure of adeno-associated virus serotype 8, a gene therapy vector, J Virol 81, 12260–12271.
Gorbatyuk, O. , Harrison, J. , Opie, S. , and Muzyczka, N. (2004) Adeno-associated virus type 2 VP2 capsid protein is nonessential and can tolerate large peptide insertions at its N terminus, J Virol 78, 6595–6609. 23. , and Chapman, M. S. (2002) The atomic structure of adenoassociated virus (AAV-2), a vector for human gene therapy, Proc Natl Acad Sci USA 99, 10405–10410. 24. Nam, H. , Lane, M. , and Agbandje-McKenna, M. (2007) Structure of adeno-associated virus serotype 8, a gene therapy vector, J Virol 81, 12260–12271.
And Srivastava, A. (1989) Rescue and replication of the adeno-associated virus 2 genome in mortal and immortal human cells, Intervirology 30, 74–85. , and Linden, R. M. (2001) Rep-dependent initiation of adeno-associated virus type 2 DNA replication by a herpes simplex virus type 1 replication complex in a reconstituted system, J Virol 75, 10250–10258. , Stow, N. , and Heilbronn, R. (2006) Role of the herpes simplex virus helicase-primase complex during adeno-associated virus DNA replication, J Virol 80, 5241–5250.